Fibroblast Growth Factor Receptor 2 (FGFR2, CD332, Keratinocyte Growth Factor Receptor 2, KGFR)

Fibroblast growth factors (FGFs) are members of a large family of structurally related heparin binding polypeptides (17-38kD) that are potent physiological regulators of growth and differentiation for a wide variety of cells of mesodermal, ectodermal and endodermal origin. FGFs are substantially involved in normal development, wound healing and repair, angiogenesis, a variety of neurotrophic activities, in hematopoiesis as well as in tissue remodeling and maintenance. They have also been implicated in pathological conditions such as tumorigenesis and metastasis. The FGF family consists of at least seventeen members designated FGF1 through FGF17. To date, four genes encoding for high affinity cell surface FGF receptors (FGFRs) have been identified: FGFR1 [flg, cek-1], FGFR2 [bek, cek-3], FGFR3 [cek-2] and FGFR4. Multiple additional variants (isoforms) arising by alternative splicing have been reported.\n\nApplications: \nSuitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nImmunohistochemistry (trypsin, protease-digested, FFPE tissue): 2-4ug/ml. \nImmunoprecipitation: Using whole lysate of transfected cells expressing recombinant human FGFR2.\nWestern Blot : 0.25-0.5ug/ml. Detects two bands at 115 and 125kD.\nOptimal dilutions to be determined by the researcher.\n\nRecommended Positive Control: \n293T, embryonic kidney cell line\n\nRecommended Tissues:\nHuman umbilical cord, rat kidney and mouse tongue\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

F4305-16A

Size

250ul

Applications

IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 1% BSA, 15mM sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Burgess, W.H. and Maciag, T., Ann. Rev. Biochem., 58, 575 (1989). 2. Klagsburn, M., Prog. Growth Factor Res., 1, 207 (1989). 3. Givol, D. and Yayon, A., FASEB. J., 6, 3362 (1992). 4. Baird, A., et al., Curr. Opin. Neurobiol., 4, 78 (1994). 5. Yayon, A., et.al., EMBO J., 5, 1885 (1992). 6. Miki, T., et, al., Proc. Natl. Acad. Sci. USA, 89, 246(1992). 7. Johnson, D. E., et. al., Mol. Cell Biol., 11, 4627 (1991). 8. Hanneken, A., et al., Proc. Natl. Acad. Sci. USA, 91, 9170 (1994). 9. Levi, E., et. al., Proc. Natl. Acad. Sci. USA, 93, 7069 (1996). 10. Zimmer, Y., et al., J. Biol. Chem., 268, 7899 (1993). 11. Yayon, A., et al., Cell., 64, 841 (1991). 12. Aviezer, D., et al., Cell, 79, 1005 (1994). 13. Wang, J.K., et al., Mol. Cell Biol., 14, 181 (1994). 14. Friesel, R.E. and Maciag, T., FASEB J., 9, 919 (1995). 15. Hughes, S. E., et al., J. Histochem. Cytochem., 45, 1005 (1997). 16. Orr-Urtreger, A., et. al., Development., 113, 1419 (1991). 17. Johnston, C. L., et al., J. Biol. Chem., 270, 30643 (1995). 18. Galvin, B. D., et al., Proc. Natl. Acad. Sci. USA, 93, 7894 (1996).

Additional Info

Recognizes human Fibroblast Growth Factor Receptor 2. A doublet at 115-125kD is seen with whole cell lysates of transfected 293T cells, a human, embryonic kidney cell line, expressing recombinant human FGFR2. No reaction with human FGFR1 and FGFR3 is detected. Species sequence homology: mouse and rat, 92%.

Alternative Names

BEK, BFR-1, CEK3, CFD1, ECT1, JWS, K-SAM, TK14, TK25