Fibroblast Growth Factor Receptor 3 (FGFR3)

Fibroblast growth factors (FGFs) produce mitogenic and angiogenic effects in target cells by signaling through cell surface receptor tyrosine kinases. There are four members of the FGF receptor family: FGFR-1 (flg), FGFR-2 (bek, KGFR), FGFR-3 and FGFR-4. Each receptor contains an extracellular ligand binding domain, a trans- membrane domain and a cytoplasmic kinase domain (1). Following ligand binding and dimerization, the receptors are phosphorylated at specific tyrosine residues (2). Seven tyrosine residues in the cytoplasmic tail of FGFR-1 can be phosphorylated: Tyr463, Tyr583, Tyr585, Tyr653, Tyr654, Tyr730 and Tyr766. Tyrosines 653 and 654 are important for catalytic activity of activated FGFR and are essential for sig- naling (3). The other phosphorylated tyrosine residues may provide docking sites for downstream signaling components such as Crk and PLCg (4,5). Activating mutations within fibroblast growth factor receptor 3 (FGFR-3) are responsible for human skeletal dysplasias including achondroplasia and the neonatal lethal syndromes thanatophoric dysplasia types I and II (6). Several of these same FGFR-3 mutations as well as overexpression of FGFR- 3 proteins have also been identified somatically in human cancers, including multiple myeloma, bladder carcinoma and cervical cancer (7). Thus, FGFR-3 may represent a potential target for therapy.\n\nApplications: \nSuitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunofluorescence. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:1000 \nImmunoprecipitation: 1:50\nImmunohistochemistry(paraffin): 1:50. Antigen retrieval with citrate buffer required.\nImmunofluorescence: 1:200\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

F4305-18H

Size

100ul

Applications

IF, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol, 0.02% sodium azide.

P Type

Mab

Purity

Supernantant

Isotype

IgG

References

1. Powers, C.J., et al., Endocr. Relat. Cancer 7: 165-197 (2000). 2. Reilly, J.F., et al., J. Biol. Chem. 275: 7771-7778 (2000). 3. Mohammadi, M., et al., Mol. Cell. Biol. 16: 977-989 (1996). 4. Mohammadi, M., et al., Mol. Cell. Biol. 11: 5068-5078 (1991). 5. Larsson, H., et al., J. Biol. Chem. 274: 25,726-25,734 (1999). 6. Wilkie, A.O., et al., Am. J. Med. Genet. 112: 266-278 (2002). 7. Miyake, M., et al., Biochem. Biophys. Res. Commun. 362: 865-871 (2007).

Additional Info

Recognizes endogenous levels of human FGF Receptor 3 at 125, 145 and 165kD. Does not crossreact with other related family members.