FIBROBLASTS, EPITHELIAL CELLS (PE)

In peripheral blood the antibody stains myeloid cells and a very small number of lymphocytes. \nStudies upon the antigen have shown it to be sensitive to SDS, but resistant to trypsin, tunicamycin and collagenase. In immunohistological studies the antibody has also been found to bind to epithelium, myoepthelium, smooth muscle and some leukocytes. F4213-01 has been shown to be useful as a cell membrane marker to characterize chondrocyte differentiation \ngiving a positive reaction with dedifferentiated human chondrocytes, and negative with differentiated chondrocytes.\n\nApplications: \nSuitable for use in Flow Cytometry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: Neat, 1:10, Use 10ul of the suggested working dilution to label 10e6 cells in 100ul. \nOptimal dilutions to be determined by the researcher.\n\nHybridoma: \nSpleen cells from immunized BALB/c mice were fused with cells of the mouse SP2 myeloma cell line.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

F4213-01

Size

100Tests

Applications

FC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH7.4 1% BSA, 0.09% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

References

1. Fearns, C. and Dowdle, E. B. (1992) The desmoplastic response: induction of collagen synthesis by melanoma cells in vitro. Int. J. Cancer. 50:621-627. 2. Kelynack, K. J. et al. (2000) Human renal fibroblast contraction of collage I lattices is an integrin-mediated process. Nephrol. Dial. Transplant. 15:1766-1772. \n3. Van Osch, G.J.V.M. , et al. (2001) Monoclonal antibody 11-Fibrau: A useful marker to characterize chondrocyte differentiation stage. Biochem. Biophys. Res. Commun. 280:806-812.

Additional Info

Recognizes a 112kD molecule expressed on the cell surface of human fibroblasts and epithelial cells.