Fibronectin BioAssay(TM) ELISA Kit, Human

Sandwich ELISA quantitative detection kit for Human Fibronectin in cell culture supernates, serum and plasma (heparin, EDTA, citrate).\n\nFibronectin (FN) also known as LETS, is identified on the surfFN of fibroblasts by labeling with radioactive compounds or specific antibodies. Fibronectin is a 430,000-dalton dimeric glycoprotein that exists in 2 forms, termed cellular and plasma fibronectin. Cellular and plasma fibronectins are heterodimers consisting of similar but not identical polypeptides.1 These two forms of FN differ in biologic activity. Fibronectins bind cell surfFNs and various compounds including collagen, fibrin, heparin, DNA, and actin. Because fibronectin stimulates endocytosis in several systems and promotes the clearance of particulate material from the circulation, it could function in the clearance of C1q-coated material such as immune complexes or cellular debris.2 Fibronectins are involved in cell adhesion, cell motility, opsonization, would healing, and maintenance of cell shape. LETS, encoded on chromosome 8, is responsible for the LETS protein expression in humans. Because LETS has been implicated in tumorigenicity and cellular transformation, it is of interest that rearrangement or modifications in the number of chromosome 8 have been associated with certain forms of cancer.3 The standard used in this kit is isolated from human plasma with the molecular mass of 200-250kD.\n\nAssay Range:\n156pg/ml-10,000g/ml\n\nSensitivity:\n< 10 pg/ml\n\nCrossreactivity:\nThere is no detectable cross-reactivity with other relevant proteins.\n\nUniprot ID:\nP02751\n\nEntrez Gene ID:\n2335\n\nKit Components:\n96-well plate pre-coated with anti-Fibronectin, Human\nLyophilized recombinant Fibronectin, Human standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

143835

Size

96Tests

References

1. Kornblihtt, A. R.; Umezawa, K.; Vibe-Pedersen, K.; Baralle, F. E. Primary structure of human fibronectin: differential splicing may generate at least 10 polypeptides from a single gene. EMBO J. 4: 1755-1759, 1985. \n2. Bing, D. H.; Almeda, S.; Isliker, H.; Lahav, J.; Hynes, R. O. Fibronectin binds to the C1q component of complement. Proc. Nat. Acad. Sci. 79: 4198-4201, 1982. \n3. Owerbach, D.; Doyle, D.; Shows, T. B. Genetics of the large, external, transformation-sensitive (LETS) protein: assignment of a gene coding for expression of LETS to human chromosome 8. Proc. Nat. Acad. Sci. 75: 5640-5644, 1978. \n