Fibronectin, Human

Fibronectin exists in three forms: 1. Soluble dimeric form, plasma fibronectin 2. Oligomers which are transiently attached to the cell surface, cell-surface fibronectin 3. Highly insoluble fibrils in the extracellular matrix, matrix fibronectin. \n\nHuman fibronectin is an adhesive glycoprotein with a molecular weight of ~500kD found in the extracellular matrix and in plasma. Fibronectin is composed of two nearly identical polypeptide chains linked by a pair of disulfide bonds. Each subunit is organized into a variety of structural domains containing specific binding sites for cells, collagen, fibrin and glycosaminoglycans. Fibronectin also participates in complex interactions with other macromolecules to influence cellular properties including adhesion, morphology, migration, hemostasis and oncogenic transformation. The human fibronectin provided is suitable to promote the adhesion and propagation of cells in vitro when used to coat cell culture surfaces, including plasticware, glassware and microcarrier beads.\n\nSource: \nHuman plasma\n\nApplications: \nCell attachment and proliferation assays on human endothelial cells, human keratinocytes and human dermal fibroblasts\n\nMW: ~220kD by SDS PAGE (Reduced)\n\nSynthesis: \nLiver\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.

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SPECIFICATIONS

Catalog Number

F4215-18B

Size

1mg

Form

Supplied as a liquid in 0.01M Tris-Cl, 0.15M sodium chloride, pH 7.4.

Purity

(same/more than) 95% (SDS-PAGE)

References

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