Fibronectin, Human

Fibronectin (cold-insoluble globulin) is a high molecular weight, adhesive, glycoprotein found in both plasma (1) and the extracellular matrix (2). Fibronectin is composed of two peptide chains of approximately 275,000 molecular weight which are linked through two interchain disulfide bonds at the COOH-terminal end of the molecule (3). The structure of fibronectin is characterized by three different types of repeating homologous sequence units (4,5). The 45 amino acid type-I repeat constitutes the NH2- and COOH-terminal ends of the protein. The two 60 amino acid type-II segments follow the first nine type-I repeats at the NH2-terminus. The 90 amino acid type-III segments occupy the central region of the fibronectin molecule. Structural differences between plasma and cellular fibronectin (6,7) as well as between the two subunits of plasma fibronectin (8,9) have been identified. These differences likely originate due to transcriptional and posttranscriptional events involving mRNA splicing (10). \n\nThe apparent function of fibronectin is to mediate cell attachment by interacting with cell surface receptors and extracellular matrix components (10-12). Fibronectin contains an Arg-Gly-Asp-Ser (RGDS) cell attachment-promoting sequence within one of the type-III homology segments in the middle of the molecule (13). This RGDS site is recognized by specific RGDS-dependent cell receptors (13) which are members of the integrin family of proteins (14). Fibronectin binding to activated platelets (15) is mediated through the RGDS cell adhesion sequence (16). Other binding domains specific for such extracellular macromolecules as heparin, fibrin(ogen), and collagen (10-12) have been identified and may be important in the fibronectin-mediated adhesion of platelets to the extracellular matrix of endothelial cells (17). Fibrin-fibronectin complexes are stabilized by the factor XIIIa-catalyzed covalent cross-linking of fibronectin to the a chain of fibrin (18).\n\nLocalization: Plasma, 0.3mg/ml (1); extracellular matrix (2)\n\nMode of Action: Mediates cell attachment to extracellular matrix (9-11)\n\nExtinction Coefficient: E1%1cm, 280nm=14.0\n\nStructure: Heterodimer (3)\n\nPercent Carbohydrate: 4-10%, depending on source (12)\n\nStorage and Stability: Lyophilized powder may be stored at 4 degrees C. Reconstitute to nominal volume by adding sterile water and store at 4 degrees C. Reconstituted product is stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

F4215-35

Size

2mg

Form

Supplied as a lyophilized powder from 20mM Tris, 150mM sodium chloride, pH 7.4. Reconstiute with 1ml sterile dH2O.

Purity

(same/more than)95% by SDS-PAGE. Fibronectin is isolated from human plasma by thermal precipitation, ion exchange, and gelatin-agarose affinity chromatography. Purity is assessed by SDS-PAGE analysis.

References

1. Mosesson, M.W., and Umfleet, R.A., J. Biol. Chem., 245, 5728 (1970). 2. Stenman, S. and Vaheri, A., J. Exp. Med., 147, 1054 (1978). 3. Skorstengaard, K., et al., Eur. J. Biochem., 161, 441 (1986). 4. Kornblihtt, A.R., et al., EMBO J., 4, 1755 (1985). 5. Odermatt, E., et al., Proc. Natl. Acad. Sci. USA, 82, 6571 (1985). 6. Hayashi, M. and Yamada, K.M., J. Biol. Chem., 256, 11292 (1981). 7. Atherton, B.T. and Hynes, R.O., Cell, 25, 133 (1981). 8. Hayashi, M. and Yamada, K.M., J. Biol. Chem., 258, 3332 (1983). 9. Sekiguchi, K. and Hakomori, S-I., J. Biol. Chem., 258, 3967 (1983). 10. Hynes, R.O., Ann. Rev. Cell Biol., 1, 67 (1985). 11. Mosher, D.F., Ann. Rev. Med., 35, 561 (1984). 12. Rouslahti, E. Ann. Rev. Biochem., 57, 375 (1988). 13. Rouslahti, E. and Pierschbacher, M.D., Cell, 44, 517 (1986). 14. Hynes, R.O., Cell, 48, 549 (1987). 15. Plow, E.F. and Ginsberg, M.H., J. Biol. Chem., 256, 9477 (1981). 16. Ginsberg, M., et al., J. Biol. Chem., 260, 3931 (1985). 17. Houdjik, W.P.M., et al., Arteriosclerosis, 6, 24 (1986). 18. Mosher, D.F., J. Biol. Chem., 250, 6614 (1975).