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Ficolin B (Ficolin-2, Collagen/Fibrinogen Domain-containing Protein 2, Ficolin-B, Ficolin-beta, L-ficolin, Fcn2, Fcnb)

Ficolin B (Ficolin-2, Collagen/Fibrinogen Domain-containing Protein 2, Ficolin-B, Ficolin-beta, L-ficolin, Fcn2, Fcnb)

Cat no: F4499-60A

Supplier: United States Biological
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Ficolins are members of the collectin family of proteins which recognize PAMP on microbial surfaces. Human and murine ficolins differ in many aspects. Mouse has two ficolin forms, termed ficolin-A and -B. Mouse ficolin-A is the plasma protein, highly expressed in liver and spleen. Mouse ficolin-B is expressed in spleen, bone marrow, and peritoneal macrophages. It is thought to facilitate phagocytosis and is involved in the activation of complement pathways. Ficolin B is a glycosylated protein which causes it to run on a WB at larger than the predicted molecular weight of 34kD. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.25-0.5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: F4499-60A
Reactivities: Mouse
Hosts: Rat
Applications: Western Blot
Size: 25ug
Form: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
P type: Mab
Isotype: IgG2b
Purity: Purified by immunoaffinity chromatography.
References: 1. Obashi T, et al. 2004. J. Biol. Chem. 279:6534. 2. Obashi T, et al. 1998. Arch Biochem Biophys. 360:223. 3. Liu Y, et al. 2005. Mol Immunol 42:1265.
Additional info: Recognizes mouse Ficolin B.

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