FK506 Binding Protein 12, Recombinant, Human (FKBP12, FK506 BP1A)

Immunophilins are a family of soluble receptors capable of binding to one of two major immunosuppressant agents; cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP). Immunophilins function as cis-trans peptidyl-prolyl isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. Thus, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline-containing proteins. \n\nFKBP12:FK506 complexes inhibit calcineurin, a calcium/calmodulin-dependent serine/threonine phosphatase which blocks T-cell activation by preventing lymphokine gene transcription. FKBP12 also plays a role in intracellular calcium regulation by associating with three types of calcium release channel complexes, cardiac and skeletal ryanodine receptors and the inositol 1,4,5-trisphosphate receptor. In interactions with members of the TGF beta family type I receptors, FKBP12 has been shown to exert an inhibitory effect on the signaling pathway. \n\nHuman FKBP12 (FK506 binding protein 1A, transcript variant 12A) (GenBank Accession No. NM_054014), (full length) with N-terminal His tag, expressed in a Baculovirus infected Sf9 cell expression system. \n\nApplications: \nSuitable for use in testing rapamycin inhibition of mTOR. Other applications not tested. \n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

F4150-31A

Size

200ug

Form

Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF and 50% glycerol.

Purity

(same/more than) 90%

References

1. Knappe, T.A., et al., J. Mol. Biol. 368(5): 1458-1468 (2007). 2. Wear, M.A., et al., Protein Expr. Purif. 51(1): 80-95 (2007). 3. Roe, S., et al., Cell Adhes. Commun. 5: 283-296 (1998). PubMed: 9762469. 4. Koslov, E.R., et al., J. Biol. Chem. 272: 27,301-27,306 (1997). 5. Hazan, R.B., et al., J. Biol. Chem. 272: 32,448-32,453 (1997). 6. Rimm, D.L., et al., Lab. Invest. 72: 506-512 (1995).