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FKHRL1 (Forkhead Box Protein O3, AF6q21 Protein, Forkhead in Rhabdomyosarcoma-like 1, FOXO3, FOXO3A)

Cat no: F4523-10E

FKHRL1 (Forkhead Box Protein O3, AF6q21 Protein, Forkhead in Rhabdomyosarcoma-like 1, FOXO3, FOXO3A)

FKHRL1 belongs to the forkhead family of transcription factors which is characterized by a distinct forkhead domain. FKHRL1 is an embryonic transcriptional regulator involved in erythropoiesis and plays an important role in the cell cycle of megakaryocytic cells. It also has a critical role in regulation of proliferation, control of oxidative stress and triggers apoptosis by inducing the expression of genes such as the Fas ligand gene TNFSF6. It is also known to regulate cardiac myocyte size downstream of AKT signaling. FKHRL1 promotes DNA repair under low stress conditions and a program of cell death under higher level of stress thus protecting quiescent cells from oxidative stress.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:500-1:1000\nImmunohistochemistry (paraffin): 1:50-1:100\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nNIH/3T3 cells\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

F4523-10E

Size

100ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Brunet, A. et al. Science. 303:2011-2015 (2004). 2. Kops, GJPL. et al. Nature. 419:316-321 (2002).

Additional Info

Recognizes human FKHRL1. Species Crossreactivity: mouse and rat.

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