Flag (DYKDDDDK) Epitope Tag

Cat no: F4524-72Y

Supplier: United States Biological

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The recombinant protein over-expression systems with well-known epitope tags are powerful strategies in checking and purifying the interest proteins, which are expressed in certain DNA vectors containing the particular tagged oligo fragments. These short oligo fragments encode small peptides (6xHis, c-Myc, HA, DYKDDDDK) or small proteins (GST, MBP, beta-Galactosidase). The antibodies against these fusion-tags can be used to monitor and purify the fusion protein. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5-2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Catalogue number:	F4524-72Y
Hosts:	Rabbit
Applications:	Western Blot
Size:	50ug
Form:	Supplied as a liquid in PBS, 0.05% sodium azide.
P type:	Pab
Isotype:	IgG
Purity:	Purified by immunoaffinity chromatography.
References:	1. Binz, H. et al. J Nat Biotechnol 22, 575 (2004). 2. Lu, Q. et al. J Gene 200, 135 (1997). 3. Malby, R. et al. J Proteins 16, 57 (1993). 4. Kolodziej, P. et al. Methods Enzymol 194, 508 (1991). 5. Wilson, I. et al. Cell 37, 767 (1984).
Additional info:	Recognizes DYKDDDDK Tag in all species.

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Flag (DYKDDDDK) Epitope Tag

Cat no: F4524-72Y

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