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Flag (DYKDDDDK) Epitope Tag (HRP)

Flag (DYKDDDDK) Epitope Tag (HRP)

Cat no: F4524-72H

Supplier: United States Biological
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Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 1:10,000-1:50,000 Western Blot: 1:1000-1:5000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
Catalogue number: F4524-72H
Hosts: Rabbit
Applications: ELISA, Western Blot
Size: 100ug
Form: Supplied as a lyophilized powder from PBS, pH 7.2, 0.01% gentamicin sulfate 1% BSA. Labeled with horseradish peroxidase (HRP). Reconstitute with 100ul sterile ddH2O.
P type: Pab
Isotype: IgG
Purity: Purified by affinity chromatography.
References: 1. Hopp, T.P. et al. (1988) A short polypeptide marker sequence useful for recombinant protein identification and purification. Bio/Technology 6: 1204-1210. 2. Slootstra, J.W. et al. (1997) Identification of new tag sequences with differential and selective recognition properties for anti-FLAG monoclonal antibodies M1, M2 and M5. Mol. Divers. 2: 156-164.
Additional info: Recognizes DYKDDDDK when fused to either the amino or carboxyl terminus of target proteins. Shows greater binding affinity and sensitivity to fusion proteins containing the DYKDDDDK sequence. Does not crossreact with any endogenous proteins.

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