FLI1 (Friend Leukemia Integration 1)

Friend leukemia integration 1 (FLI1) transcription factor is an ETS domain-containing transcription factor that plays an important and highly conserved role in vertebrate development, particularly hematopoiesis, where it functions to activate transcription of genes that promote erythroblast proliferation (1-4). In mice, the Fli1 locus is a common retroviral insertion site for the Friend Murine Leukemia Virus (F-MuLV), such that a majority of F-MuLV-induced erythroleukemias are associated with aberrant Fli1 expression (5). Notably in humans, aberrant FLI1 expression has also been linked to poor prognosis in acute myeloid leukemia (6). Also in humans, a t(11;22)(q24;q12) chromosomal translocation has been described that generates a chimeric protein (EWS/FLI1) comprised of the amino-terminal transactivation domain of Ewing Sarcoma breakpoint region 1 (EWS) and the carboxy-terminal ETS domain of FLI1 (7). The EWS/FLI1 fusion protein functions as a transcriptional activator that is reportedly responsible for >85% of the known cases of pediatric Ewing

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

137435

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, NHP

Form

Supplied in 10mM sodium HEPES, pH 7.5, 150mM NaCl, 100ug/ml BSA and 50% glycerol.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Spyropoulos, D.D. et al. (2000) Mol Cell Biol 20, 5643-52. 2. Pimanda, J.E. et al. (2007) Proc Natl Acad Sci U S A 104, 17692-7. 3. Jagadeeswaran, P. et al. (2010) Blood Cells Mol Dis 44, 175-80. 4. Tijssen, M.R. et al. (2011) Dev Cell 20, 597-609. 5. Ben-David, Y. et al. (1990) Proc Natl Acad Sci U S A 87, 1332-6. 6. Kornblau, S.M. et al. (2011) Blood 118, 5604-12. 7. May, W.A. et al. (1993) Proc Natl Acad Sci U S A 90, 5752-6. 8. Braun, B.S. et al. (1995) Mol Cell Biol 15, 4623-30. 9. Riggi, N. and Stamenkovic, I. (2007) Cancer Lett 254, 1-10.

Additional Info

Recognizes endogenous levels of total human FLI1. Species Crossreactivity: mouse, rat, monkey.