FLIP, CT, (FLICE Inhibitory Protein) Blocking Peptide

Control Peptide for F4550\n\nApoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3 protease family. Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP, respectively1,2. The human FLIP was also cloned by several labs independently and termed Casper, I-FLICE, FLAME-1, CASH and CLARP3-7. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and -10, and potently inhibits apoptosis induced by all known death receptors. Four splice variants of c-FLIPs have been identified and termed FLIPa, b, g, and d, respectively.\n\nApplications: \nSuitable for use in Antibody Blocking. Other applications not tested.\n\nRecommended Dilution:\nAntibody Blocking: equal volume of antibody for 30 minutes at 37 degrees C usually completely blocks the antibody activity in Western Blot..\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

F4550P

Size

50ug

Form

Supplied as a liquid in PBS, pH 7.2, .01% BSA, 0.02% sodium azide.

References

1. Irmler, M. Thome, M, Hahne M, Schneider P, Hofmann K, Steiner V, Bodmer JL, Schroter M, Burns K, Mattmann C, Rimoldi D, French LE, Tschopp J. Inhibition of death receptor signals by cellular FLIP. Nature 388:190-195 (1997).