Flt3 (Fms-like Tyrosine Kinase 3, FLT-3, CD135, FL Cytokine Receptor, FLK2, Fms-related Tyrosine Kinase 3, Stem Cell Tyrosine Kinase 1, STK1, STK-1, Tyrosine-protein Kinase Receptor FLT3)

FMS-related tyrosine kinase 3 (FLT3, also called Flk2), is a member of the type III receptor tyrosine kinase family, which includes c-Kit, PDGFR and M-CSF receptors. FLT3 is expressed on early hematopoietic progenitor cells and supports growth and differentiation within the hematopoietic system. FLT3 is activated after binding with its ligand FL, which results in a cascade of tyrosine autophosphorylation and tyrosine phosphorylation of downstream targets. The p85 subunit of PI3 kinase, SHP2, GRB2 and Shc are associated with FLT3 after FL stimulation . Tyr589/591 is located in the juxtamembrane region of FLT3 and may play an important role in regulation of FLT3 tyrosine kinase activity. Somatic mutations of FLT3 consisting of internal tandem duplications (ITDs) occur in 20% of patients with acute myeloid leukemia.\n\nApplications:\nSuitable for use in ELISA, Immunoprecipitation, Immunohistochemistry and Western Blot. Other applications not tested. \n\nRecommended Dilutions: \nImmunoprecipiation: 1:100\nImmunohistochemistry (Paraffin): 1:100\nWestern Blot: 1:1000; Bands at 130 and 160kD. 130kD - Non-glycosylated form; 160kD - Mature, glycosylated form.\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

F4600-07B

Size

100ul

Applications

ELISA, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol and less than 0.02% sodium azide.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Shurin, M.R., et al., Cytokine Growth Factor Rev. 9: 37-48 (1998). 2. Naoe, T., et al., Cancer Chemother. Pharmacol. 48(1): S27-S30 (2001). 3. Namikawa, R., et al., Stem Cells 14: 388-395 (1996). 4. Beslu, N., et al., J. Biol. Chem. 271: 20,075-20,081 (1996). 5. Zhang, S. and Broxmeyer, H.E., Biochem. Biophys. Res. Commun. 277: 195-199 (2000). 6. Zhang, S., et al., J. Leukoc. Biol. 65: 372-380 (1999). 7. Mizuki, M., et al., Blood 96: 3907-3914 (2000).

Additional Info

Recognizes human Flt3 Ligand. Detects endogenous levels of FLT3 protein. Does not crossreact with related proteins. Species Crossreactivity: mouse.