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Fluorescein Isothiocyanate (FITC) (AP)

Cat no: F5249-01

Fluorescein Isothiocyanate (FITC) (AP)

Fluorescein isothiocyanate (FITC) is a small ?uoresent molecule that is commonly conjugated to antibodies for immuno?uorescent cell labeling. In conjunction with anti-FITC antibodies, it can also be used as an epitope tag for detection and signal ampli?cation.\n\nApplications: \nSuitable for use in Immunoblot and Enzyme Immunoassays.\nOther applications have not been tested.\n\nRecommened Dilution:\nWestern Blot: 1:10-1:100 (Recommended substrate: BCIP).\nELISA: 1:100-1:500 (Recommended substrate: p-NPP ).\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

F5249-01

Size

1ml

Applications

ELISA, WB

Hosts

Rabbit

Form

Supplied as a liquid in 0.05M Tris buffer, pH 8, 10mg/ml BSA, 0.1% sodium azide. Labeled with Alkaline Phosphatase (AP).

P Type

Pab

Purity

Immunoaffinity purified, absorbed antibody covalently coupled to highly purified alkaline phosphatase (Bovine). Free enzyme is removed.

Isotype

IgG

Additional Info

Recognizes fluorescein. No crossreactivity with immunoglobulins of any species tested. Solid phase hapten-antibody capture.

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