Follistatin 288 (FSTL1, FS288)

Follistatin (FST) is a single-chain glycosylated protein of gonadal origin, originally identified as an antagonist of activin and suppressor of follicle stimulating hormone (FSH) synthesis/secretion. Alternative splicing of FST mRNA generates the two isoforms FS315 and FS288, the latter of which is the main cell-surface form and binds with high affinity to surface heparan sulphate proteoglycans, while FS315 binds with only low-affinity, and is considered to be the main circulating form of follistatin. As well as activin, FST interacts with other growth factors such as bone morphogenetic proteins (BMPs) -2, -4 and -7, myostatin, and growth differentiation factor 9 (GDF-9), playing a key role in cellular differentiation/proliferation, embryonic development and tissue repair. \n\nApplications:\nSuitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (paraffin): Antigen retrieval using heat treatment prior to staining of paraffin sections is required. Sodium citrate buffer, pH 6.0 is recommended for this purpose. Optimal dilutions to be determined by the researcher.\n\nHybridoma: \nSpleen cells from immunized Balb/c mice were fused with cells of the Sp2/0 myeloma cell line.\n \nPositive Control: \nTestis\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. \n

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SPECIFICATIONS

Catalog Number

F9090-03

Size

100ug

Applications

ELISA, IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Evans, L.W. et al. (1998) Development, validation and application of an ultra-sensitive two-site enzyme immunoassay for human follistatin. J. Endocrinol. 156: 275-282 2. McPherson, S.J. et al. (1999) Expression of activin A and follistatin core proteins by human prostate tumor cell lines. Endocrinology. 140: 5303-5309. 3. Majdic, G. et al. (1997) Testicular expression of inhibin and activin subunits and follistatin in the rat and human fetus and neonate and during postnatal development in the rat. Endocrinology. 138: 2136-2147.

Additional Info

Recognizes human follistatin isoform 288 (FS288). Displays 10% crossreactivity with FS315 in ELISA.