Folate Receptor 1 (FOLR1), also known as Folate Receptor alpha and Folate Binding Protein (FBP), is a 37-42kD protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown. Mature FOLR1 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage. Mouse FOLR1 shares 83% and 93% aa sequence identity with human and rat FOLR1, respectively. FOLR1 is predominantly expressed on epithelial cells and is dramatically upregulated on many carcinomas. It is critically required during early embryogenesis as shown in knockout mice which die in utero with gross morphological defects. FOLR1 is internalized to the endosomal system where it dissociates from its ligand before recycling to the cell surface. A soluble form of FOLR1 can be proteolytically shed from the cell surface into the serum and breast milk.
Source:
Recombinant corresponding to aa1-232 from mouse FOLR1 fused to 6-His-tag at C-terminal expressed in Chinese Hamster Ovary cell line, CHO.
Molecular Weight:
~25.3kD
Biological Activity:
Measured in a competitive binding assay. When Folic Acid-Bovine Serum Albumin is immobilized at 5ug/ml (100ul/well), Recombinant Mouse (rm) FOLR1 inhibits 50% binding of biotinylated rmFOLR1(7.9nM) at the concentration range of 30-120nM.
Endotoxin: ~1EU/1ug (LAL)
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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