FOPflash (mutant Tcf binding sites) (TK-luciferase reporter) Negative Control

Cat no: F5950

Supplier: United States Biological

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Transfection grade T cell factor (Tcf) reporter plasmid containing two full and one incomplete copy of the Tcf binding site (mutated) followed by three copies in the reverse orientation, upstream of the Thymidine Kinase (TK) minimal promoter and Luciferase open reading frame. This plasmid serves as a negative control to TOPflash which contains wild type Tcf binding sites. Size: 5.5kb Applications: Suitable for use in TCF activity assays. Storage and Stability: For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Catalogue number:	F5950
Size:	5ug
Form:	Supplied as a liquid in 10mM Tris-HCl, pH 8.0, 1mM EDTA
Purity:	Purified by immunoaffinity chromatography.
References:	1. Lu, D., et al., Proc. Natl. Acad. Sci. USA 101: 3118-23, 2004. 2. Haq, S., et al., Proc. Natl. Acad. Sci. USA, 100: 4610-5, 2003. 3. Leung-Hagesteijn, C., et al., Embo. J. 20: 2160-70, 2001. 4. Coghlan, M. P., et al., Chem. Biol. 7: 793-803, 2000. 5. Ishitani, T., et al., Nature 399: 798-800. 1999. 6. Korinek, V., et al., Mol. Cell. Biol. 18: 1248-1256, 1998.

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FOPflash (mutant Tcf binding sites) (TK-luciferase reporter) Negative Control

Cat no: F5950

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