Formate BioAssay(TM) Kit (Formic Acid, HCOOH)

Formic acid (HCOOH) is the simplest carboxylic acid and occurs naturally, notably in ant and bee venom. It is normally present at low levels in blood and urine (up to ~0.5mM) but can be present in concentrations as high as 5mM in acute methanol poisoning. Environmental exposure to formaldehyde can also elevate the blood and urine levels of formate.\n\nSample Type:\nCell and tissue culture lysates, urine, plasma and serum, as well as many other biological fluids.\n\nIntended Use:\nThe kit provides a convenient means for detecting formate in biological samples such as in serum or plasma, cells, culture and fermentation media. There is no need for pretreatment or purification of samples.\n\nRange:\n0.5-10nmols/well\n\nSpecificity:\nMammalian\n\nTest Principle:\nIn the Formate Assay Kit, formate is oxidized to generate a product resulting in color formation (l=450nm) proportional to formate concentration.\n\nKit Components:\nFormate Assay Buffer: 1x25ml\nFormate Enzyme Mix: 1x1vial\nFormate Substrate Mix: 1x1vial\nFormate Standard (100 mM): 1x100ul\n\nStorage and Stability:\nStore components at 4 degrees C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts