Formiminotransferase Cyclodeaminase, Recombinant, Human

Formiminotransferase cyclodeaminase, a bifunctional enzyme of tetrahydrofolate synthesis, is the target antigen of anti-LC1 (liver cytosol antigen type 1) autoantibodies. Presence of LC1 autoantibodies is a marker for type 2 autoimmune hepatitis (for which anti-LKM 1/cytochrome P450 2D6 autoantibodies are a further marker). Serum LC1 autoantibody concentrations appear to fluctuate in parallel with aminotransferase levels, a particularly intriguing observation that suggests a possible role of LC1 autoreactivity in the pathogenic mechanism leading to hepatocyte injury.\n\nDescription: \nRecombinant Human Formiminotransferase Cyclodeaminase 1 produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 59,749 dalton. Recombinant LC-1 is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.\n\nImmunological functions:\n1. Binds IgG-type human auto-antibodies.\n2. Standard ELISA test (checker-board analysis of positive/negative sera panels, immuno-dot test).\n\nApplications: \nWestern-Blot with monoclonal anti-hexa-His-tag antibody.and autoimmune hepatitis (AIH II) patient sera.\n\nCoating Concentration:\n0.35-0.7ug/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.\n\nStorage Conditions: \nStore at 4 degrees C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degrees Cfor longer periods of time. Please avoid freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

F6002-20

Size

5ug

Form

Supplied as a liquid in 16mM HEPES buffer, pH 7.6, 240mM sodium chloride and 20% glycerol.

Purity

(same/more than) 95% (SDS-PAGE)

References

1. Localization of Golgi 58K protein (formiminotransferase cyclodeaminase) to the centrosome.\n\nHistochem Cell Biol 2006 Mar 14;\n\n2. Structure of the bifunctional and Golgi-associated formiminotransferase cyclodeaminase octamer.\n\nEMBO J 2004 Aug 4;23(15):2963-71\n\n3. Characterization of the antigenicity of the formiminotransferase-cyclodeaminase in type 2 autoimmune hepatitis.\n\nExp Cell Res 2004 Jan 15;292(2):332-41\n\n4. Distinct epitopes on formiminotransferase cyclodeaminase induce autoimmune liver cytosol antibody type 1.\n\nHepatology 2001 Sep;34(3):494-501\n\n5. The crystal structure of the formiminotransferase domain of formiminotransferase-cyclodeaminase: implications for substrate channeling in a bifunctional enzyme.\n\nStructure 2000 Jan 15;8(1):35-46\n\n6. Crystallization and preliminary X-ray analysis of the formiminotransferase domain from the bifunctional enzyme formiminotransferase-cyclodeaminase.\n\nActa Crystallogr D Biol Crystallogr 1999 Jun;55(Pt 6):1206-8