FOXP3 (Forkhead Box P3, AIID, DIETER, IPEX, JM2, PIDX, SCURFIN, XPID)

FOXP3 (a 381aa protein) is a member of the forkhead/winged-helix family of transcriptional regulators and is highly conserved across mammals. FOXP3 is essential for normal immune homeostasis. FOXP3 is stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, at a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene and a more specific marker of regulatory T cells than other T cells.\n\nApplications: \nSuitable for use in Flow Cytometry, Western Blot and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: Band is seen at ~48kD in FOXP3 transfected and CD25/CD4 positive regulatory T cell lysates.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nMouse CD4+CD25+ T cells\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

F9050-10E

Size

100ul

Applications

FC, ICC, WB

Hosts

Rabbit

Reactivities

Mouse

Form

Supplied as a liquid in Tris-glycine, sodium chloride, 0.05% sodium azide.

P Type

Pab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG

References

1. Lim, H.W., et al., J. Immunol. 175: 4180-4183 (2005). 2. Polanczyk, M.J., et al., J. Immunol. 173: 2227-2230 (2004). 3. Ochs, H.D., et al., Immunol. Rev. 203: 156-164 (2005).

Additional Info

Recognizes mouse FOXP3 (CD4+CD25+T cells). Does not react with human Jurkat or mouse CD4+CD25-T cell lysates.

Alternative Names

MGC141961, MGC141963