Fractalkine/CX3CL1 BioAssay(TM) ELISA Kit, Human

Sandwich ELISA quantitative detection kit for Human Fractalkine/CX3CL1 in cell culture supernates, serum and plasma (heparin, EDTA), saliva, urine and human milk.\n\nChemokine (C-X3-C motif) ligand 1 (CX3CL1) is a large cytokine protein of 373 amino acids, it contains multiple domains and is the only known member of the CX3C chemokine family. It is also commonly known under the names fractalkine (in humans) and neurotactin (in mice).1, 2 CX3CL1 is produced as a long protein (with 373-amino acid in humans) with an extended mucin-like stalk and a chemokine domain on top. The mucin-like stalk permits it to bind to the surface of certain cells. However a soluble (90kD) version of this chemokine has also been observed. Soluble CX3CL1 potently chemoattracts T cells and monocytes, while the cell-bound chemokine promotes strong adhesion of leukocytes to activated endothelial cells, where it is primarily expressed.2 CX3CL1 elicits its adhesive and migratory functions by interacting with the chemokine receptor CX3CR1.3 Its gene is located on human chromosome 16 along with some CC chemokines known as CCL17 and CCL22.2, 4 It can act as a mediator of smooth muscle cell migration in human atherosclerosis, rather than mediate inflammatory cell recruitment.5 \n\nAssay Range:\n156pg/ml-10,000pg/ml\n\nSensitivity:\n< 50 pg/ml\n\nCrossreactivity:\nThere is detectable cross-reactivity with Eotaxin, MCP-1, MCP-2, 6Ckine, MARC < 0.2%.\n\nUniprot ID:\nA0N0N7\n\nEntrez Gene ID:\n6376\n\nKit Components:\n96-well plate pre-coated with anti-Fractalkine/CX3CL1, Human\nLyophilized recombinant Fractalkine/CX3CL1, Human standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

143839

Size

96Tests

References

1. Pan et al. Neurotactin, a membrane-anchored chemokine upregulated in brain inflammation. Nature 387: 611-617, 1997.\n2. Bazan et al. A new class of membrane-bound chemokine with a CX3C motif. Nature 385: 640-644, 1997.\n3. Imai et al. Identification and molecular characterization of fractalkine receptor CX3CR1, which mediates both leukocyte migration and adhesion. Cell 91: 521-530, 1997.\n4. Nomiyama et al. Human chemokines fractalkine (SCYD1), MDC (SCYA22) and TARC (SCYA17) are clustered on chromosome 16q13. Cytogenet. Cell Genet. 81: 10-11, 1998.\n5. Lucas, A. D.; Bursill, C.; Guzik, T. J.; Sadowski, J.; Channon, K. M.; Greaves, D. R. : Smooth muscle cells in human atherosclerotic plaques express the fractalkine receptor CX3CR1 and undergo chemotaxis to the CX3C chemokine fractalkine (CX3CL1). Circulation 108: 2498-2504, 2003.\n