Fractalkine, Recombinant, Human, (CX3CL1)

Fractalkine, also named neurotactin, is a novel chemokine recently identified through bioinformatics. Fractalkine has a unique C-X3-C cysteine motif near the amino-terminus and is the first member of a fourth branch of the chemokine superfamily. Unlike other known chemokines, fractalkine is a type 1 membrane protein containing a chemokine domain tethered on a long mucin-like stalk. Human fractalkine cDNA encodes a 397 amino acid (aa) residue membrane protein with a 24 aa residue predicted signal peptide, a 76 aa residue chemokine domain, a 241 aa residue stalk region containing 17 degenerate mucin-like repeats, a 19 aa residue transmembrane segment and a 37 aa residue cytoplasmic domain. The extracellular domain of human fractalkine can be released, possibly by proteolysis at the dibasic cleavage site proximal to the membrane, to generate soluble fractalkine. The soluble chemokine domain of human fractalkine was reported to be chemotactic for T cells and monocytes while the soluble chemokine domain of mouse fractalkine was reported to chemoattract neutrophils and T-lymphocytes but not monocytes.\n\nBiological Activity: \nFully biologically active when compared to standard. Determined by its ability to chemoattract human T-Lymphocytes using a concentration range of 5.0-10.0 ng/ml.\n\nAA Sequence: \nQHHGVTKCNITCSKMTSKIPVALLIHYQQNQASCGKRAIILETRQHRLFCADPKE\nQWVKDAMQHLDRQAAALTRNG\n\nEndotoxin: (same/less than)1EU/ug as determined by LAL method.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Reconstitute with distilled water, containing 0.1% BSA . Aliquot and store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

F6069-95C

Size

5ug

Form

Supplied as a lyophilized powder from PBS, pH 7.54 Reconstitute in sterile Reconstitute in sterile ddH2O or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1mg/ml.

Purity

>97% by SDS-PAGE and HPLC analyses.