Frizzled 10, Fc Chimera, Recombinant, Human (FZD10)

Frizzled 10 is a 68kD seven pass transmembrane glycoprotein in the Frizzled family of Wnt receptors. The 205 amino acid N-terminal extracellular region of Frizzled 10 contains a cysteine-rich domain that comprises the Wnt binding domain and mediates receptor oligomerization. The C-terminal cytoplasmic tail contains a PDZ-interaction motif. PDZ motifs mediate intracellular binding to scaffolding proteins. Within the cysteine-rich domain, human Frizzled 10 shares 71% amino acid (aa) sequence identity with Frizzled 9 and 31%-46% with Frizzled 1, -2, -3, -4, -5, -6, -7 and -8. It shares 96%, 94%, 90%, and 82% aa sequence identity with chick, mouse, Xenopus and zebrafish Frizzled 10, respectively. Frizzled 10 is expressed during embryogenesis in the primitive streak, dorsal neural tube, developing brain, limb bud and airway epithelium. It is induced by Shh and co-localizes with Shh and Wnt-7a in the neural tube. In the adult, Frizzled 10 is expressed in placenta, gastric glands and colon and renal tubule epithelial cells. Frizzled 10 associates with LRP5 to transduce Wnt-7a and Wnt-7b signals, resulting in the stabilization of cytoplasmic beta-catenin. Frizzled 10 is also upregulated in some cancers and transformed cell lines. It binds hypoxia inducible gene 2, which promotes oncogenic Wnt signaling and functions as an autocrine growth factor for renal cell carcinomas.\n\nSource:\nA DNA sequence encoding human Frizzled 10 (Ile21-Gly161; Accession # Q6NSL8) was fused to IgG1 (Pro100-Lys330) at the C-terminal, via a linker peptide (IEGRMD) expressed in NS0 cells.\n\nN-terminal Sequence Analysis: \nIle21\n\nPredicted Molecular Mass: \n43kD (Monomer)\n\nSDS-PAGE: \n56-58kD, reducing conditions\n\nActivity: \nMeasured by its binding ability in a functional ELISA. Biotinylated recombinant mouse Wnt-5a immobilized on a streptavidin-coated plate at 1ug/ml can bind recombinant human Frizzled 10, Fc Chimera with an apparent Kd <35nM.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

F6401-47

Size

50ug

Form

Supplied as a lyophilized powder from PBS, pH 7.4. \nReconstitute with sterile PBS to 0.1mg/ml. Endotoxin: (same/less than)1EU/ug (LAL)

Purity

(same/more than)90% by SDS-PAGE under reducing conditions and visualized by silver stain.

References

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