Frizzled5 Fc Chimera, Recombinant Human

Frizzled5 is an ~62kD seventransmembrane (7TM) glycoprotein that is one of at least ten Frizzled family Wnt receptors (1 3). Frizzled proteins are thought to be G proteincoupled\n(1). Wnt engagement of Frizzled, with low density lipoprotein receptorrelated proteins LRP5\nor LRP6 acting as coreceptors, stabilizes B-catenin and promotes gene transcription that is important in development and tissue maintenance (1). LRP independent or пїЅnoncanonicalпїЅ\nFrizzled signaling pathways also exist (1). Human Frizzled5 cDNA encodes 585 amino\nacids (aa) including a 26 aa signal peptide, a 212 aa extracellular domain (ECD), the 7TM region (aa 213 521), and a Cterminal cytoplasmic domain with a PDZ binding motif (aa 522 585) (2, 5). The ECD includes a cysteinerich region (CRD, aa 29 156) that binds Wnts and is highly conserved among Frizzleds (1, 2, 4). Human Frizzled5 shares 95%, 95% and 96% aa identity with mouse, rat and canine Frizzled5, respectively, within the Nterminal extracellular portion. It also shares 82% aa identity with human Frizzled8. Frizzled5 is expressed in\nembryonic tissues (telencephalon, pituitary, thalamus, hypothalamus, eye, liver, spleen, lung\nand kidney), in the adult retina, colon and pancreatic islets, some cancer cell lines, human embryonic stem cells and in some monocyte and lymphocyte populations (2 9). It is required for ongoing survival of neurons in the parafascicular nucleus of the mouse thalamus (5). Null mutations show a role for Frizzled5 in maintenance of yolk sac and placental vasculature (6). Deletion specifically in the eye results in persistence of vitreous vasculature that normally regresses during late development (7, 10). In macrophages, Frizzled5 participates in a CaMKII signaling pathway that promotes inflammation (8, 9). Wnts implicated as ligands for Frizzled5 include Wnt5a, Wnt9b, Wnt10b, Wnt2b, and Wnt 7a (5, 6, 11, 12).\n\nSource: \nRecombinant corresponding to human, Frizzled5 Fc Chimera, NпїЅterminus.\n\nMolecular Weight: \n~50.1kD\n\nBiological Activity:\nMeasured by its binding ability in a functional ELISA. In a 100ul reaction mixture containing biotinylated rmWntпїЅ5a at 100 ng/ml and rhFrizzledпїЅ5/Fc Chimera dilutions, the concentration of recombinant, human FrizzledпїЅ5/Fc Chimera that produces 50% of the maximal binding response is found to be approximately 5пїЅ20ng/ml. Optimal concentrations should be determined by each laboratory for each application.\n\nEndotoxin Level:\n<1EU/ug (LAL method)\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

F6401-04G

Size

50ug

Applications

ELISA

Form

Supplied as a lyophilized powder in PBS, pH7.2. Reconstitute with 200ug/ml PBS.

Purity

~95% (SDS-PAGE)

References

1. Schulte, G. and V. Bryja (2007) Trends Pharmacol. Sci. 28:518. 2. Saitoh T. et al. (2001) Int. J. Oncol. 19:105. 3. Wang, Y. et al. (1996) J. Biol. Chem 271:4468. 4. Katoh, Y. and M. Katoh (2007) Int. J. Oncol. 30:751. 5. Liu, C. et al. (2008) J. Neurosci. 28:5641. 6. Ishikawa, T. et al. (2001) Development 128:25.\n7. Burns, C.J. et al. (2008) Dev. Dyn. 237:1614. 8. Periera, C. et al. (2008) Arterioscler. Thromb. Vasc. Biol. 28:504. 9. Blumenthal, A. et al. (2006) Blood 108:965. 10. Zhang, J. et al. (2008) Invest. Ophthalmol. Vis. Sci. 49:5561. 11. He, X. et al. (1997) Science 275:1652. 12. Caricasole, A. et al. (2003) J. Biol. Chem 278:37024.