FUBI, Recombinant, Human (Ubiquitin-like Protein Finkel-Biskis-Reilly Murine Sarcoma Virus, MNSF beta, 40S Ribosomal Protein S30, asr1, FAU, Fub1, RPS30, S30)

The Ubiquitin-like Protein Finkel-Biskis-Reilly Murine Sarcoma Virus (FUBI), also known as Monoclonal Nonspecific Suppressor Factor beta (MNSF beta), is a 74 amino acid (aa) Ubiquitin-like protein modifier with a predicted molecular weight of 8kD that is encoded by the FAU gene. FUBI/MNSF beta is translated as a fusion protein with the ribosomal protein S30; the fusion protein is post-translationally cleaved into two functional proteins. The mouse and rat FUBI/MNSF beta-ribosomal protein S30 fusion orthologs share 97% and 98% aa sequence identity with the human protein, respectively. Like many Ubiquitin-like proteins, FUBI/MNSF beta contains a C-terminal Gly-Gly motif and can be covalently conjugated to acceptor proteins to form adducts that modulate protein action. For example, FUBI/MNSF beta conjugation to Bcl-G inhibits the ERK1/ERK2 cascade downstream of TLR4 activation in macrophage cell lines. Additionally, FUBI/MNSF beta conjugation to Endophilin II has been shown to regulate Dectin-1-mediated phagocytosis and inflammation. The role of FUBI/MNSF beta downstream of TLR2 signaling is unclear, however novel FUBI/MNSF beta adducts may contribute to TLR2 signaling, as silencing of FUBI/ MNSF beta enhanced signaling via a TLR2-specific ligand.\n\nSource:\nRecombinant protein corresponding to human FUBI/MNSF beta.\n\nMolecular Weight: \n~7.8kD\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

145791

Size

250ug

Form

Supplied as a liquid in 50mM HEPES, pH 8.0, 100mM NaCl, 1mM DTT.

Purity

~95% (SDS-PAGE)

References

1. Rossman,T.G. et al. (2003) Oncogene 22:1817-1821. 2. Nakamura, M. & Omura, S. (2008) Biosci. Biotech. Biochem. 72:1915-1920. 3. Nakamura, M. & Yamaguchi, S. (2006) J. Biol. Chem. 281:16861-16969. 4. Nakamura, M & Watanabe, N. (2010) Biochem, Biophys. Res. Comm. 15: 275- 61. 5. Nakamura, M. et al. (2012) Mol. Cell Biochem. 364:39-43.