Home  >  Products  >  FXY2 (Probable E3 Ubiquitin-protein Ligase MID2, Midin-2, Midline Defect 2, Midline-2, RING Finger Protein 60, Tripartite Motif-containing Protein 1, MID2, RNF60, TRIM1)
FXY2 (Probable E3 Ubiquitin-protein Ligase MID2, Midin-2, Midline Defect 2, Midline-2, RING Finger Protein 60, Tripartite Motif-containing Protein 1, MID2, RNF60, TRIM1)

FXY2 (Probable E3 Ubiquitin-protein Ligase MID2, Midin-2, Midline Defect 2, Midline-2, RING Finger Protein 60, Tripartite Motif-containing Protein 1, MID2, RNF60, TRIM1)

Cat no: F9400-02A

Supplier: United States Biological
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MID2 is a member of the tripartite motif (TRIM) family. The MID motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to microtubular structures in the cytoplasm. Its function has not been identified. Alternate splicing of the gene encoding MID2 results in two transcript variants encoding different isoforms. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1-3ug/ml Immunohistochemistry (paraffin): 5-10ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Mouse Heart Lysate Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: F9400-02A
Reactivities: Human, Mouse, Non-Human Primate
Hosts: Goat
Applications: Immunohistochemistry, Western Blot
Size: 100ug
Form: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: 1. Buchner G, Montini E, Andolfi G, Quaderi N, Cainarca S, Messali S, Bassi MT, Ballabio A, Meroni G, Franco B. MID2, a homologue of the Opitz syndrome gene MID1: similarities in subcellular localization and differences in expression during development. Hum Mol Genet. 1999 Aug;8(8):1397-407. PMID: 10400986.
Additional info: Recognizes human FXY2. Species Crossreactivity: monkey and mouse.

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