Fyn (FYN, SLK, SYN, MGC45350, FYN oncogene related to SRC, FGR, YES, src-like kinase, c-syn protooncogene, tyrosine kinase p59fyn(T), src/yes-related novel gene, OKT3-induced calcium influx regulator, proto-oncogene tyrosine-protein kinase fyn)

Alternate Name: FYN, SLK, SYN, MGC45350, FYN oncogene related to SRC, FGR, YES, src-like kinase, c-syn protooncogene, tyrosine kinase p59fyn(T), src/yes-related novel gene, OKT3-induced calcium influx regulator, proto-oncogene tyrosine-protein kinase fyn\n\nDomain Information: Eukaryotic protein kinase domain; SH3 domain; Serine/Threonine protein kinases, catalytic domain; Src homology 2 domains; Src homology 3 domains; Src homology domain 2; Tyrosine kinase, catalytic domain\n\nGene Ontology Terms: learning; feeding behavior; protein kinase cascade; protein phosphorylation; protein tyrosine kinase\n\nApplication(s):\nPeptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 55-60kD band observed in Jurkat, A431 and NIH-3T3 lysates (predicted MW of 62kD according to NP_002028.1). Recommended for use at 0.2-1ug/ml.\n\nStaining 0.2ug/ml) of Jurkat lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nPeptide Blocking:\nCorresponding peptide is available for Peptide Blocking studies. See F9500-03C-P. Antibody

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SPECIFICATIONS

Catalog Number

F9500-03C

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

Semba K, Nishizawa M, Miyajima N, Yoshida MC, Sukegawa J, Yamanashi Y, Sasaki M, Yamamoto T, Toyoshima K., yes-related protooncogene, syn, belongs to the protein-tyrosine kinase family., Proc Natl Acad Sci U S A 1986 Aug;83(15):5459-63

Additional Info

This antibody is expected to recognize all three reported isoforms (as represented by NP_002028.1; NP_694592.1; NP_694593.1). Species Crossreactivity: human, mouse, rat.