G-CSF Receptor (Granulocyte Colony-stimulating Factor Receptor, G-CSF-R, CD114, Csf3r, Csfgr)

Granulocyte colony stimulating factor is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic and granulocyte lineage. G-CSF plays an important role in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis. Cell activation by G-CSF is mediated by granulocyte colony stimulating factor receptor alpha (G-CSF R; also CD114), a 95-105kD type I transmembrane protein and member of the cytokine receptor superfamily, type I cytokine receptor family, and type 2 subfamily of receptor proteins. Mouse G-CSF R is synthesized as an 837aa precursor that contains a 25aa signal sequence, a 601aa extracellular domain a 24aa transmembrane region, and a 187aa cytoplasmic tail. The ECD contains one Ig-like C2-type domain, five fibronectin type-III domains, and 11 potential sites for N-linked glycosylation. Within the ECD there is also a WSXWS motif aa319-323 that is necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. Also, within the cytoplasmic domain there is a Box 1 motif which is required for JAK interaction and/or activation. Mouse G-CSF R shares 63% aa sequence identity with human G-CSF R. G-CSF R is expressed in mature neutrophils, neutrophilic precursors, myeloid leukemia cells, and placenta. Mutations have been found in the gene encoding G-CSF R in some patients with severe congenital neutropenia. These mutations typically lead to a truncation in the cytoplasmic domain of the G-CSF R leading to maturation arrest of neutrophilic precursors in the bone marrow and neutropenia in peripheral blood. Binding of G-CSF to its receptor induces dimerization or oligomerization of the receptor activating cytoplasmic tyrosine kinases. Signal transduction from pathways that involve Janus tyrosine kinases/signal transducer and activator of transcription proteins (Jak1, Jak2, and Tyk2/STAT3 and STATG), src-related protein tyrosine kinases (Lyn and Syk), Ras/MAP kinase, and phosphatidylinositol have been reported to be activated upon G-CSF stimulation.\n\nApplications:\nSuitable for use in Neutralization Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nEndotoxin: ~0.01EU/1ug (LAL)\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

G8950-50P

Size

500ug

Hosts

Rat

Reactivities

Mouse

Form

Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.

P Type

Mab

Purity

Purified by Protein A/G affinity chromatography.

Isotype

IgG2B

References

1. Ward, A.C. (2007) Front. Biosci. 12:608. 2. Layton, J.E. and N.E. Hall (2006) Front. Biosci. 11:3181. 3. Mitsui, T. et al. (2003) Blood 101:2990. 4. Nicola, N.A. in Cytokine Reference, 2001, Oppenheim, J.J. and M. Feldmann, eds. Academic Press p.1935.

Additional Info

Recognizes mouse G-CSF R. Does not react with recombinant human G-CSF R, rhGM-CSF R beta, recombinant mouse GM-CSF R alpha, or rmM-CSF R.