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G3BP-1, phosphorylated (Ser232) (Ras GTPase-Activating Protein-Binding Protein 1, GAP SH3 Domain-Binding Protein 1, ATP-dependent DNA helicase VIII, HDH-VIII, G3BP1, G3BP)

Cat no: G0875-01E

G3BP-1, phosphorylated (Ser232) (Ras GTPase-Activating Protein-Binding Protein 1, GAP SH3 Domain-Binding Protein 1, ATP-dependent DNA helicase VIII, HDH-VIII, G3BP1, G3BP)

G3BP are scaffolding proteins linking signal transduction to RNA metabolismand belongs to a evolutionarily conversed family throughout eukaryota. G3BP exists in three isoforms G3BP1, 2a and 2b which are the products of two distinct genes. The proteins are relatively ubiquitously expressed. G3BP1 plays an potential role in cell cycle regulation. G3BP1 also bind and regulate cmyc which is an important transcription factorpredominantly involved in the regulation of cell cycle progression. The protein acts as a major DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA:DNA and RNA:RNA duplexes in an ATP-dependent fashion. Over-expression of G3BP1 has been observed in a range of human tumors, including breast, head and neck, colon and thyroid.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:500-1:1000\nImmunohistochemistry (paraffin): 1:50-1:100\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\n293 cells\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

G0875-01E

Size

50ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Katharine, I. et al. Int. J. Dev. Biol. 48:1065-1077 (2004).

Additional Info

Recognizes human G3BP-1, phosphorylated at Ser232.

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