GABA A receptor delta subunit

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA Receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six as, four bs and four gs, plus alternative splicing variants of some of these subunits, have been identified (4, 5, 8). Injection in oocytes or mammalian cell lines of cRNA coding for a- and b-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a g-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different a-subunits of the receptor (2, 3, 4, 6). More recently there have been a number of studies demonstrating that the a-subunit of the receptor may affect subunit assembly (1) and may also confer differential sensitivity to neurosteroids and to ethanol (7).\n\nApplications: \nSuitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nImmunoprecipitation: 10ul per 50ug lysate\nImmunohistochemistry (Frozen): 1:250\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

G1015-19N3

Size

100ul

Applications

IHC, IP, WB

Hosts

Rabbit

Reactivities

Mouse, Rat

Form

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

P Type

Pab

Purity

Purified by affinity chromatography.

References

1. Korpi ER, et al. (2002) Neurosci 109:733-743. 2. McKernan RM, et al. (2000) Nature Neurosci 3:587-592. 3. Mehta AK, Ticku MK (1998) Mol Brain Res 67:194-199. 4. Ogris W, et al. (2004) Biochem Pharmacol 68:1621-1629. 5. Olsen RW, Tobin AJ (1990) FASEB 4:1469-1480. 6. P

Additional Info

Recognizes the 52kD delta subunit of rat brain GABA-A. No known reactivity with other GABA subunits. No homology with other GABA subunits. Species crossreactivity: mouse