GABA Transporter 2 (GAT-2, (Gamma aminobutyrIc acid)

GABA is a major inhibitory neurotransmitter and the GABAergic transmission is terminated by the rapid Na+/Cl-dependent uptake of through GABA transporters. It has been subdivided into neural and glial uptake systems on the basis of pharmacological properties. Recently, molecular cloning studies have identified multiple subtypes of GABA transporters (GAT1, GAT2, GAT3; and betaine GABA transporter (BGT-1). There is ~50% homology between various GABA transporter subtypes. GABA transporters are predicted to contain 12 potential transmembrane domains. The NH2 and COOH-termini are predicted to be intracellular. Two of the high affinity (Km~10 uM) rat GABA transporters (GAT2 and GAT3/GAT-B) share higher amino acid identity (68% and 65%, respectively) with the kidney betaine transporter than with GAT-1 (52% AA identity). GAT1 and GAT3 have been detected in various parts of the brain while GAT2 is found in many tissues. It appears that GAT1 and GAT3 are involved in distinct GABAergic transmission while GAT2 may be important in non-neural functions.\n\nApplications: \nSuitable for use in Immunohistochemistry and Western Blot. Other applications have not been tested. \n\nRecommended Dilutions: \nImmunohistochemistry: 1:500-1:750 on 4% paraformaldehyde fixed tissue. It is recommended that the antibody is diluted in PBS containing 10% normal goat serum and 0.5% Triton X-100.\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G1015-70

Size

10ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Mouse, Rat

Form

Supplied as a lyophilized powder from 0.1M PBS, 0.1% sodium azide. Reconstitute with 20ul sterile ddH2O.

P Type

Pab

Purity

Purifed by affinity chromatography.

Isotype

IgG

References

1) J. Comp. Neurology (1996) 375:212

Additional Info

Recognizes the C-terminus of mouse GABA Transporter-2 (GAT-2), Does noto crossreactivity to the C-termini of other transmitter transporters detected. Species crossreactivity: rat.