GABRA1 (GABA(A) receptor subunit alpha-1)

GABRA1, Gamma-aminobutyric acid receptor subunit alpha-1, is a protein that in humans is encoded by the GABRA1 gene. The 1,055-bp GABRA1 clone contained an open reading frame and 260 nucleotides in the 5-prime noncoding region. The 351-amino acid sequence shows 97% homology with its bovine counterpart. GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified.\n\nUniProt Number:\nP14867\n\nGene ID:\nGABRA1\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry (Paraffin).\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Handling:\nStore at -20?C for one year. After reconstitution, store at 4?C for one month. Can also be aliquoted and stored frozen at -20?C for long term.\nAvoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

144556

Size

100ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1.Cossette, P., Liu, L., Brisebois, K., Dong, H., Lortie, A., Vanasse, M., Saint-Hilaire, J.-M., Carmant, L., Verner, A., Lu, W.-Y., Wang, Y. T., Rouleau, G. A.Mutation of GABRA1 in an autosomal dominant form of juvenile myoclonic epilepsy.Nature Genet. 31: 184-189, 2002.\n2.Kralic, J. E., Criswell, H. E., Osterman, J. L., O'Buckley, T. K., Wilkie, M. E., Matthews, D. B., Hamre, K., Breese, G. R., Homanics, G. E., Morrow, A. L.Genetic essential tremor in gamma-aminobutyric acid-A receptor alpha-1 subunit knockout mice.J. Clin. Invest. 115: 774-779, 2005.\n3.Rudolph, U., Crestani, F., Benke, D., Brunig, I., Benson, J. A., Fritschy, J.-M., Martin, J. R., Bluethmann, H., Mohler, H.Benzodiazepine actions mediated by specific gamma-aminobutyric acid-A receptor subtypes.Nature 401: 796-800, 1999.\n

Additional Info

Recognizes human, mouse and rat GABRA1 . No crossreactivity with other proteins.