gAcrp30, Recombinant, Mouse (Globular Adipocyte Complement-related Protein of 30kD, apm-1, Adipolean)

Murine gAcrp30 is a 16kD protein originating from a proteolytic cleavage of Acrp30 (adipocyte complement-related protein of 30kD). Research shows that gAcrp30 is more active than the full length of Acrp30. gAcrp30 is expressed and secreted by adipose tissue. gAcrp30 possesses unique pharmacological properties for regulation of body weight and lipid metabolism. It is a key component in controlling energy homeostasis. Recombinant Murine gAcrp30 is a 16kD protein consisting of 145 amino acid residues.\n\nAA Sequence: \nMKGEPGEAAY MYRSAFSVGL ETRVTVPNVP IRFTKIFYNQ QNHYDGSTGK FYCNIPGLYY FSYHITVYMK DVKVSLFKKD KAVLFTYDQY QEKNVDQASG SVLLHLEVGD QVWLQVYGDG DHNGLYADNV NDSTFTGFLL YHDTN\n \nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Reconstitute to nominal volume by adding sterile 5mM Tris, pH 7.6. Aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nCountry of Origin: USA

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SPECIFICATIONS

Catalog Number

G1016-03

Size

5ug

Form

Supplied as a lyophilized powder in 5mM Tris, pH 7.6.\nReconstitute with 5mM Tris, pH 7.6 to a concnentration of 0.1-1mg/ml.

Purity

(same/more than) 98% by SDS-PAGE and HPLC. Endotoxin: (same/less than) 0.1ng/ug (1EU/ug)

References

1. Rakatzi, I., Diabetologia 47(2): 249-58 (2004, Feb). 2. Hu, X.B., et al., ACTA BIOCHIMICA BIOPHYSICA SINICA 35(11): 1023-1028 (2003). 3. Tomas, E., et al., PNAS USA 99(25): 16309-13 (2002, Dec 10). 4. Tsao, T.S., et al., J. Biol. Chem. 277(33): 29359-62 (2002, Aug 16). 5, Fruebis, J., et al., PNAS USA 98(4): 2005-10 (2001, Feb 13).

Additional Info

Biological Activity: Determined by its abiliyt to inhibit the proliferation of murine myeloid cell lines M1. The ED50 for this effect is (same/less than) 15ug/ml.