Galactokinase, Recombinant, Human (Galactokinase 1, GALK1, Galactose Kinase)

Galactokinase is a major enzyme for the metabolism of galactose and its deficiency causes congenital cataracts in the adult population. GALK1 sequence shares the greatest level of conservation, 44.5% identity with that from E. coli and 34.6% amino acid identity with the product of the human GALK2 gene.\n\nSource: \nRecombinant human galactokinase, full length, with N-terminal His tag, expressed in E. coli (NM_000154).\n\nSpecific Activity: ~200pmoles/min/ug\n\nAssay Conditions: \nA 50ul Galactokinase reaction is conducted in buffer containing 20mM Tris, pH 7.4, 5mM MgCl2, 50mM sodium chloride, 5uM ATP, 1mM DTT and 1mM substrate D-Galactose at RT for 1 hour. ATP reduction is detected using Kinase-Glo Luminescent Kinase Assay Platform.\n\nApplications: \nUseful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested.\n\nRecommended Dilution: \nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.

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