The B-Bridge Galactose Colorimetric Detection Kit (cat.# K3042-1) quantitatively measures galactose present in serum, EDTA and heparin plasma, buffers, and tissue culture media (TCM). Detection of galactose is species independent.
A D-(+)-galactose standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Samples are mixed with the Colorimetric Substrate and horseradish peroxidase and the reaction initiated by addition of galactose oxidase. The reaction is incubated at room temperature for 30 minutes. The galactose oxidase reacts with galactose to produce hydrogen peroxide which, in the presence of HRP, reacts with the colorless Colorimetric Substrate to produce a pink-colored product which is read at 560 nm. Increasing levels of galactose cause a linear increase in color.
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