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Galanin (GAL1, GALN, GLNN)

Cat no: G1043-01B

Galanin (GAL1, GALN, GLNN)

Galanin is a 3kD (predicted), secreted member of the Galanin family of peptides. It is co-expressed with differing neuropeptides in a variety of neuron cell types. Galanin affects multiple metabolic processes by binding to one of three GPCRs. GalR1 blocks insulin secretion, GalR2 initiates neurogenesis and GalR3 influences addictive behavior. The human Galanin proform is 11-12kD in size (predicted) and 104aa in length. Proteolytic processing generates a 30aa mature Galanin peptide (aa33-62), plus a phosphorylated (Ser117), C-terminal 59aa GMAP fragment that is apparently involved in the processing of noxious stimuli. Once secreted, Galanin can undergo additional proteolytic degradation. Over aa20-123, the human Galanin proform shares 72% aa identity with mouse Galanin proform. \n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1ug/ml\nELISA: 0.5-1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS, sterile 40-50% glycerol, aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G1043-01B

Size

100ug

Applications

ELISA, WB

Hosts

Sheep

Reactivities

Hum

Form

Supplied as a lyophilized powder from 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with sterile PBS, sterile 40-50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

Additional Info

Recognizes human Galanin. Species sequence homology: mouse (72%).

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