Galectin-3 (Gal-3, 35kD Lectin, Carbohydrate-binding Protein 35, CBP 35, Galactose-specific Lectin 3, Galactoside-binding Protein, GALBP, IgE-binding Protein, L-31, Laminin-binding Protein, Lectin L-29, Mac-2 Antigen, LGALS3, MAC2)

Galectin-3 (galactose-specific lectin 3) is a 30-32kD glycoprotein, also known as Mac-2, Gal-3, RL-29, galactose-specific lectin 3, CBP-35, L-34, and eBP. It is a member of the animal B-galactoside-binding lectin family that is expressed in the cell nucleus, cytoplasm, plasma membrane, or extracellular matrix by variety of tumor cells, monocytes/macrophages, epithelial cells, Kupffer cells, and dendritic cells. Galectin-3 contains carbohydrate recognition domains and binds to B-galactoside residues bearing glycoproteins, such as as IgE, IgA, galactose, casein kinase I, laminin, mucin, LAMPs, and CD66. Galectin-3 is an adhesion molecule and plays an important role in regulation of cell proliferation, differentiation and tumor cell metastasis. Structurally, Galectin-3 possesses NWGR motif that is conserved in the BH-1 domain of the BCL-2 family and functions as an apoptotic molecule.\n\nApplications:\nSuitable for use in Immunofluorescence, Flow Cytometry and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: (same/less than)0.5ug/10e5 cells in 100ul\nWestern Blot: 0.5-1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

G1044-50J

Size

500ug

Applications

FC, IF, WB

Hosts

Mouse

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

P Type

Mab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG1,k

References

1. Kim, H-R. C., et al. 1999. Cancer Res. 59:4148. 2. Reljic, R., et al. 2004. Immunol. Lett. 93:51. 3. Takenaka, Y. et al. 2004. Mol. and Cell. Biol. 24:4395. 4. Joo, GH., et al. 2001. J. Leukoc. Biol. 69:555.

Additional Info

Recognizes human Galectin-3. Species Crossreactivity: mouse.