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(gamma)-CEHC EIA Kit (plasma and serum)

Cat no: 10010621

(gamma)-CEHC EIA Kit (plasma and serum)

The (gamma)-tocopherol metabolite, (gamma)-CEHC, is produced in the liver by the action of CYP450 enzymes and excreted in urine at levels that exceed all other tocopherol metabolites. In plasma, (gamma)-tocopherol has the ability to trap NO radicals, and both (gamma)-tocopherol and (gamma)-CEHC inhibit COX activity in macrophages and epithelial cells. Thus, the concentration of (gamma)-tocopherol in plasma could be indicative of events related to cardiovascular disease and cancer. Cayman's (gamma)-CEHC EIA is a competitive assay that can be used for efficient quantification of (gamma)-CEHC in plasma and serum.

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SPECIFICATIONS

Catalog Number

10010621

Form

96 Well

P Type

Assay Kits

Weight

0

Storage Temp

-20

Shipping Temp

-20

Additional Info

Tocopherols and tocotrienols are forms of vitamin E that have antioxidative properties. .gamma.-Tocopherol is the most abundant form of vitamin E in the diet. Its metabolite, .gamma.-CEHC (2,7,8-trimethyl-2-(.beta.-carboxyethyl)-6-hydroxychroman), is produced in the liver by the action of cytochrome P450 enzymes and excreted in urine at levels that exceed all other tocopherol metabolites. In plasma, .gamma.-tocopherol has the ability to trap nitric oxide radicals, and both .gamma.-tocopherol and .gamma.-CEHC inhibit cyclooxygenase activity in macrophages and epithelial cells. Thus, the concentration of .gamma.-tocopherol in plasma could be indicative of events related to cardiovascular disease and cancer. Cayman's .gamma.-CEHC EIA Kit (plasma and serum) is a competitive assay that can be used for efficient quantification of .gamma.-CEHC in plasma and other sample matrices. The EIA typically displays an IC50 (50% B/B0) of approximately 900 pg/ml and a detection limit (80% B/B0) of approximately 150 pg/ml.

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