GDNF (Astrocyte-derived trophic factor)

GDNF, Glial cell-derived neurotrophic factor, is a protein that, in humans, is encoded by the GDNF gene. GDNF is a small protein that potently promotes the survival of many types of neurons. GDNF is a founding member of the GDNF family of ligands (GFL).The GDNF gene is mapped to human chromosome 5p13.1-p12 by fluorescence in situ hybridization (FISH). This gene encodes a highly conserved neurotrophic factor. The recombinant form of this protein was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosisof motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene.\n\nUniProt Number:\nP39905\n\nGene ID:\nGDNF\n\nApplications:\nSuitable for use in Western Blot, Immunohistochemistry (Paraffin) and Immunocytochemistry.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Handling:\nStore at -20?C for one year. After reconstitution, store at 4?C for one month. Can also be aliquoted and stored frozen at -20?C for long term.\nAvoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

144567

Size

100ug

Applications

ICC, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Iwashita, T., Kruger, G. M., Pardal, R., Kiel, M. J., Morrison, S. J.Hirschsprung disease is linked to defects in neural crest stem cell function.Science 301: 972-976, 2003.\n2. Lin, L.-F. H., Doherty, D. H., Lile, J. D., Bektesh, S., Collins, F.GDNF: a glial cell line-derived neurotrophic factor for midbrain dopaminergic neurons.Science 260: 1130-1132, 1993.\n3. Schindelhauer, D., Schuffenhauer, S., Gasser, T., Steinkasserer, A., Meitinger, T.The gene coding for glial cell line derived neurotrophic factor (GDNF) maps to chromosome 5p12-p13.1.Genomics 28: 605-607, 1995.\n

Additional Info

Recognizes human, mouse and rat GDNF. No crossreactivity with other proteins.