GFI1B, CT (Zinc Finger Protein Gfi-1b, Growth Factor Independent Protein 1B, Potential Regulator of CDKN1A Translocated in CML)

GFI1b and its homolog GFI1 are transcriptional repressors and important regulators of erythroid and megakaryocytic development and differentiation (1,2). GFI1b negatively regulates transcription by recruiting chromatin regulatory proteins including CoREST, the histone demethylase LSD1 and HDACs 1 and 2, which associate with GFI1b via its SNAG repression domain (3). GFI1b has also been shown to control the differentiation of erythroid and megakaryocytic progenitors by regulating TGF-B signaling at the bipotent progenitor stage (4). Inactivation of GFI1b in mice leads to embryonic lethality due to failure to produce functional erythrocytes and megakaryocytes (2). The GFI1b gene locus can be autoregulated by binding to its own promoter in hematopoietic cells, likely through interacting with GATA-1, another transcription factor essential for erythroid and megakaryocytic development (5). Mutations in GFI1b are implicated in various leukemias (6) and GFI1b has been found in a complex with GATA-1 and SUZ12 on repressed genes in erythroleukemia cells (7).\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

G2032-67

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, NHP

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, <0.02% sodium azide, 50% glycerol.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1.Duan, Z. and Horwitz, M. (2003) Hematology 8, 339-44. 2.Saleque, S. et al. (2002) Genes Dev 16, 301-6. 3.Saleque, S. et al. (2007) Mol Cell 27, 562-72. 4.Randrianarison-Huetz, V. et al. (2010) Blood 115, 2784-95. 5.Vaziri, K. et al. (2005) South Med J 98, 825-6. 6.Elmaagacli, A.H. et al. (2007) Br J Haematol 136, 212-9. 7.Yu, M. et al. (2009) Mol Cell 36, 682-95.

Additional Info

Recognizes endogenous levels of total human GFI1b. Species Crossreactivity: Monkey, mouse and rat.