Glucose-6-Phosphate BioAssay(TM) Kit

Supplier:

United States Biological

Cat no: G3053-25

Glucose-6-Phosphate BioAssay(TM) Kit

Glucose-6-phosphate (G6P) is a key sugar intermediate for glucose to get into cells, and then enter either metabolic pathways or storage. G6P can enter the glycolytic pathway, the pentose phosphate shunt or be stored as glycogen or starch. G6P is utilized by its dehydrogenase to generate reducing equivalents in the form of NADPH. This is particularly important in red blood cells where a G6PDH deficiency leads to hemolytic anemia. \n\nThe Glucose-6-phosphate Assay Kit is a simple, sensitive and rapid means of quantifying G6P in a variety of samples. Sample types include: cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids. In the assay, glucose-6-phosphate is oxidized with the generation of a product which is utilized to convert a nearly colorless probe to an intensely colored product with an absorbance at 450 nm. The Glucose-6-phosphate Assay Kit can detect G6P in the range of 1 to 30 nmoles with detection sensitivity of ~10uM of G6P.\n\nKit Components:\nG3053-25A: G6P Assay Buffer, 1x25ml. Allow to warm to RT before use.\nG3053-25B: G6P Enzyme Mix, 1x1vial (green cap; lyophilized)\nG3053-25C: G6P Substrate Mix 1x1vial (red cap; lyophilized)\nG3053-25D: G6PStandard 1x 10umol. 1x1vial (yellow cap; lyophilized)\n\nStorage and Stability:\nStore the kit at -20 degrees C, protect from light. Stable for at least 6 months. For maximum recovery of product, centrifuge the small vials after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

G3053-25

Size

100Tests

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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