Glucose Regulated Protein 94 (Grp94, gp96, ECGP, HSP90b1, TRA1)

Grp94 (glucose regulated protein 94, gp96) is a constitutively expressed endoplasmic reticulum (ER) lumenal protein that is up-regulated in response to cellular stress such as heat shock, oxidative stress or glucose depletion. Grp94 is thought to play a role in protein translocation to the ER, in their subsequent folding and assembly, and in regulating protein secretion (1). Grp94 also plays a role in antigen presentation by accessing the endogenous pathway and eliciting specific CTL responses to chaperone bound peptides via MHC class I pathway (2). Grp94 is a member of the Hsp90 family of stress proteins and shares sequence homology with its cytosolic equivalent, Hsp90 (3). Both Hsp90 and Grp94 are calcium binding proteins (4). Despite sharing 50% sequence homology over its N domains and complete conservation in its ligand binding domains with Hsp90, Grp94 and Hsp90 differ in their interactions with regulatory ligands as Grp94 has weak ATP binding and hydrolyisis activity (5). Grp94 exists as a homodimer and the two subunits interact at two distinct intermolecular sites, C terminal dimerization domains and the N-terminal interacts with the middle domain of opposing subunits. (6). Grp94 contains a carboxy terminal KDEL (Lys-Asp-Glu-Leu) sequence which is believed to aid in its retention in the ER (7).\n\nApplications: \nSuitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:10,000\nImmunoprecipitation: 1:80\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

136781

Size

25ug

Applications

ICC, IHC, IP, WB

Hosts

Rabbit

Form

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. Rudolph R.W., and Bedows E. (1997) J Biol Chem 272: 3125-3128. 2. Srivastava P.K., et al. (1994) Immunogenetics. 39(2):93-98. 3. Mazzarella R.A., and Green M. (1987) J Biol Chem 262: 8875-8883. 4. Kang, H.S. and Welch W.J. (1991) J Biol Chem 266(9): 5643-5649. 5. Soldano K.L., et al. (2003) J Biol Chem 278(48): 48330-48338. 6. Chu F., et al. (2006) Protein Sci 15(6): 1260-1269. 7. Peter F., et al., (1992) J Biol Chem 267: 10631-10637. 8. Allen S. et al. (2000) Blood 96(2): 560-568. 9. Sato K et al. (2001) Blood 98(6): 1852-1857. 10. Yun S.-W. et al (2000) Brain Research Bulletin.52(5): 371-378. 11. Choukhi A., et al. (1998) J. Virol. 72: 3851-3858. 12. Hoshino T., et al. (1998) Blood 91(11): 4379-4386. 13. Riera M. et al. (1999) Mol. Cell Biochem. 191: 97-104. 14. Gusarova V., et al. (2001) J. Biol. Chem. 276(27): 24891-24900.

Additional Info

Recognizes mouse GRP94. Species Crossreactivity: human, rat, bovine.

Alternative Names

Grp94, gp96, ECGP, HSP90b1, TRA1