Glutamate receptor 5/1 (metabotropic)

Glutamate is the main excitatory neurotransmitter in the brain. For many years it had been considered to act only on the ligand-gated receptor channels-termed NMDA, AMPA and kainate receptors that are involved in the fast excitatory synaptic transmission. Recently, glutamate has been shown to regulate enzymes producing second messengers via specific receptors coupled to G-proteins. These receptors are called metabotropic glutamate receptors. In expression systems, Group-I receptors, stimulate phospholipase C as revealed by an increase in phosphoinositide turnover, and Ca2+ release from internal stores. Group-II and -III receptors are coupled to the inhibition of adenylyl cyclase. The Group-I receptors include mGluR1a and mGluR5. The Group-II receptors in include mGluR2 and mGluR3.\n\nApplications: \nSuitable for use in Western Blot and Immunohistochemistry (Fr). Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G3500-31

Size

100ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Rat

Form

Tris- glycine

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Pin, j-P, Duvoisin, R. Review: Neurotransmitter receptor 1: The metabotropic glutamate receptors: structure and function. Neuropharmacology. 34: 1-26, 1995.

Additional Info

Specific for metabotropic glutamate receptor 5 (mGluR5/1). This antibody crossreacts with mGluR1a. Species Crossreactivity: reacts with rat mGluR5/1. Other species have not been tested, but should work with most mammalian species due to sequence homology.