Glutathione S Transferase pi (GSTpi)

GST's are responsible for the metabolism of a broad range of xenobiotics and carcinogens. The enzyme catalyzes the reaction of glutathione with a wide variety of organic compounds to form thioethers, a reaction that is sometimes a first step in a detoxification process leading to mercapturic acid formation. GSTs play an important role defense against electrophilic chemicals generated by cellular oxidative reactions catalyzed by cytochrome P450 and other oxidases. GSTs are believed to defend against the highly reactive electrophiles that are formed as a result of cellular oxidative processes. Based on amino acid sequence similarities and antibody cross-reactivities, the mammalian cytosolic GSTs are divided into the following classes: alpha, mu, kappa, theta, pi, zeta, omega and sigma. GSTs exists as homodimers of ~25kD. GST A1-1 is mainly found in kidney, intestine, lung, and liver. GST M1-1 is restricted to liver and few other tissues. GST P1-1 has a widespread distribution in most tumor cells and tissues but it is absent in liver.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:10,000-1:100,000. Coat ELISA plates with 50-100ng/well GST pi. \nWestern Blot: 1:500-1:3000 (ECL) \nNote: Actual dilution of antibody will vary depending upon the type of technique used to detect primary antibody such colorimetric or chemiluminescence substrates. The nature and concentration of GST in may also affect antibody concentration.\nOptimal dilutions to be determined by the researcher.\n\nWestern Blot Positive Control: \nG8135-26D\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

G8135-26H

Size

50ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as a liquid in PBS, 0.05% sodium azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Ali-Osman, F., J. Biol. Chem. 272: 10004-10012 (1997). 2. Board, P., J. Biol. Chem. 268: 15655-15658 (1993). 3. Colin, J., Henderson, C., Wolf, R., Kitteringham, N., Powell, H., Otto, D., Park. B.K.: Increased resistance to acetaminophen hepatotoxicity in mice lacking glutathione S-transferase Pi. PNAS 97: 12741-12745 (2000). 4. Konohana, A., J. Invest. Derm. 95: 119-126 (1990).

Additional Info

Recognizes rat GST pi. Species sequence homology: mouse, human.