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Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH, GAPD, HGNC:4141, G3PD, MGC88685)

Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH, GAPD, HGNC:4141, G3PD, MGC88685)

Cat no: G8140-16A

Supplier: United States Biological
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Glyceraldehyde-3-phosphate dehydrogenase catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. A GAPD pseudogene has been mapped to Xp21-p11 and 15 GAPD-like loci have been identified. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: G8140-16A
Reactivities: Human, Canine
Hosts: Goat
Applications: ELISA, Western Blot
Size: 100ug
Form: Supplied as a liquid in Tris-saline, pH 7.2. 0.5% BSA, 0.02% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
Alternative names: GAPDH, GAPD, HGNC:4141, G3PD, MGC88685
References: Ismail SA, Park HW. . Structural analysis of human liver glyceraldehyde-3-phosphate dehydrogenase.. Acta Crystallogr D Biol Crystallogr. 2005 Nov;61(Pt 11):1508-13. Epub 2005 Oct 19. PMID: 16239728 [PubMed - in process]
Additional info: GAPDH is constitutively expressed in almost all tissues at high levels. It is therefore a useful marker when a loading/positive control is required in western blotting. Species Crossreactivity: Human. Other Species have not been tested. Expected crossreactivity based upon sequence homology: Human, Mouse, Rat, canine, etc

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