Glycerol Kinase, Recombinant, E. coli, aa1-502 (GK, GK1, GKD, glpK, Glycerokinase)

Supplier:

United States Biological

Cat no: 145796

Glycerol Kinase, Recombinant, E. coli, aa1-502 (GK, GK1, GKD, glpK, Glycerokinase)

Glycerol kinase from E. coli (glpK) catalyzes the ATP-dependent phosphorylation of glycerol to produce sn-glycerol-3-phosphate (G3P), the first and rateпїЅlimiting step in the utilization of glycerol (1). In the presence of glycerol, glpK is stimulated by interaction with the membrane-bound glycerol facilitator (2). In the presence of glucose, glpK activity is allosterically inhibited by fructose-1,6пїЅbisphosphate (FBP) of the glycolytic pathway (3). Under physiological conditions, the enzyme is in an equilibrium between the active dimer and the inactive tetramer. FBP binds to and stabilizes the inactive form, therefore shifting the usage of glycerol metabolic pathway to glycolytic pathway (4). glpK is also inhibited by phosphocarrier protein IIAGlc by a regulatory mechanism distinct from that of FBP (5). GlpK is a member of a superfamily of ATPases that includes actin, hexokinase and the heat shock protein hsc70. Although these proteins are dissimilar in amino acid sequence and function, they share similar tertiary folds and likely the same catalytic mechanism. The enzyme activity was measured using a phosphatase-coupled kinase assay (6).\n\nSource:\nRecombinant protein corresponding to aa1-502 from E. coli Glycerol Kinase, fused at 6-his tag at C-terminal, expressed in E. coli.\n\nMolecular Weight:\n~52-55kD\n\nEndotoxin:\n<1.0EU/1ug (LAL method)\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

145796

Size

20ug

Form

Supplied as a liquid in Tris, NaCl, Glycerol, DTT. BSA free.

Purity

~75% (SDS-PAGE)

References

1. Pettigrew, D.W. et al. (1988) J. Biol. Chem. 263:135. 2. Voegele, R.T. et al. (1993) J. Bacteriol. 175:1087. 3. Applebee, M.K. et al. (2011) J. Biol. Chem. 286: 23150. 4. Hurley, J.H. et al. (1993) Science 259:673. 5. Feese, M.D. et al. (1998) Structure 6:1407. 6. Wu, Z.L. (2011) PLoS ONE 6(8): e23172.

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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