Because of an alanine for serine substitution, this peptide is not a substrate for glycogen synthase kinase 3, but instead serves as a negative control. The peptide is similar to skeletal muscle glycogen synthase; it contains sites 3b and 3c. Site 4, however, is replaced with alanine.
This peptide is a negative control for assay of G8170-46 activity. G8170-26, but not this lot of GS-2 (Ala21) peptides
Sequence: YRRAAVPPSPSLSRHSSPHQAEDEEE.
Applications:
Suitable for use in Protein Phosphorylation Assays. Other applications have not been tested.
Recommended Dilutions:
Protein Phosphorylation Assays: Use at 20-250uM final concentration. Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Reconstitute to nominal volume by adding ddH2O. Aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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