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Glycogen Synthase Peptide 2 [Ala21]

Cat no: G8170-25


Supplier: United States Biological
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Because of an alanine for serine substitution, this peptide is not a substrate for glycogen synthase kinase 3, but instead serves as a negative control. The peptide is similar to skeletal muscle glycogen synthase; it contains sites 3b and 3c. Site 4, however, is replaced with alanine. This peptide is a negative control for assay of G8170-46 activity. G8170-26, but not this lot of GS-2 (Ala21) peptides Sequence: YRRAAVPPSPSLSRHSSPHQAEDEEE. Applications: Suitable for use in Protein Phosphorylation Assays. Other applications have not been tested. Recommended Dilutions: Protein Phosphorylation Assays: Use at 20-250uM final concentration. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Reconstitute to nominal volume by adding ddH2O. Aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: G8170-25
Size: 250ug
Form: Lyophilized powder.
Purity: (same/more than) 97% by reverse phase HPLC
References: 1. Sutterland, et al., Biochem J. 296: 15, 1993. 2 Sutterland, et al., FEBS Lett. 338: 37, 1994.3. Woodgett, J.R., Semin. Cancer Biol. 5: 269, 1994.

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