Glypican 6 (Glypiated Proteoglycan 6)

Glypican 6 (glypiated proteoglycan 6) belongs to the family of membrane heparin sulfate proteoglycans that are linked to the cell membrane by a glycosylphosphatidylinositol (GPI) anchor. The glucosaminoglycans (GAGs) attachment sites of glypican family proteins are localized close to the cell membrane in the C-terminal region. Glypicans are thought to function as low-affinity binding proteins that retain signaling molecules (cytokines, growth factors, proteases) at the cell surface. Mature human and mouse glypican share 98% amino acid sequence identity. Glypican 6 is a putative cell surface coreceptor for growth factors, extracellular matrix proteins, proteases and anti-proteases.\n\nApplications:\nSuitable for use in Flow Cytometry, Western Blot and Direct ELISA. Other applications not tested.\n\nRecommended Dilutions:\nFlow Cytometry: 2.5ug/10e6 cells using HepG2 human hepatocellular carcinoma cells.\nWestern Blot: 1ug/ml using human Glypican 6\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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