Home  >  Products  >  GNPDA1 (Glucosamine-6-phosphate Deaminase 1, Glucosamine-6-phosphate Isomerase 1, GlcN6P Deaminase 1, Oscillin, GNPI, HLN, KIAA0060)
GNPDA1 (Glucosamine-6-phosphate Deaminase 1, Glucosamine-6-phosphate Isomerase 1, GlcN6P Deaminase 1, Oscillin, GNPI, HLN, KIAA0060)

GNPDA1 (Glucosamine-6-phosphate Deaminase 1, Glucosamine-6-phosphate Isomerase 1, GlcN6P Deaminase 1, Oscillin, GNPI, HLN, KIAA0060)

Cat no: G8252-02E

Supplier: United States Biological
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GNPDA1 is an allosteric enzyme catalyzing the reversible conversion of D-glucosamine-6-phosphate into D-fructose-6-phosphate and ammonium, a reaction that under physiological conditions proceeds to the formation of fructose-6-phosphate. It is the sole enzyme linking hexosamine systems with glycolytic pathways by transferring hexosamines from structural macromolecules into the glycolytic pathway. GNPDA1 localizes close to the developing acrosome vesicle and in spermatozoa close to the acrosomal region, and may play a role in the acrosome reaction. Human GNPDA1 gene is localized to chromosome 5q21. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.5-1ug/ml Immunohistochemistry (formalin fixed paraffin embedded): 10ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Human, mouse and rat spleen lysate Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: G8252-02E
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Immunohistochemistry, Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by Protein A affinity chromatography.
References: 1. Arreola, R. et al. FEBS Lett. 551:63-70 (2003). 2. Herman, W. et al. The FASEB Journal. 12:91-99 (1998).
Additional info: Recognizes human GNPDA1. Species Crossreactivity: mouse and rat. Species sequence homology: bovine.

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